Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. For instruments equipped with a 561 nm Yellow-Green laser, a 585/15 nm filter is recommended for detection. When making decisions about which fluorochromes to use in your experiments, you'll want to know their relative emission spectra. APC (Allophycocyanin) spectrum - APC (Allophycocyanin) is ... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 605nm. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Figure 1: Tandem dyes A. Diagrammatic representation of transfer of energy between donor and acceptor molecules in a tandem dye PE-Cy7. BD Horizon™ BUV395 is a polymer-based dye with an emisson max at 395 nm. Please review our cookies policy and configure your cookies for your experience. The absorption spectrum of a typical fluorochrome is illustrated in Figure 1(a) where the relative intensity of absorption is plotted against the measured wavelength. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. A tandem dye consists of a … APC has a 650-nanometer wavelength absorption maximum and a 660-nanometer fluorescence emission maximum. BUV661 has been exclusively developed by BD Life Sciences. PE-Cy7 conjugated reagents are as bright as PE conjugates. Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. Due to nearly identical excitation and emission properties, APC and Alexa Fluor® 647 cannot be used simultaneously. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. BD Horizon™ Red 718 (R718) is a small molecule fluorochrome with an emission max near 718 nm. Not for resale. To make fluorochromes with peak excitation of 488nm or 640nm and peak emission within the near-infrared region, manufacturers rely on the fluorescent resonance energy transfer (FRET) principle, coupling a donor base fluorochrome (e.g. Not for use in diagnostic or therapeutic procedures. Due to nearly identical excitation and emission properties but different spillover characteristics, APC-Cy7 and APC-H7 cannot be used simultaneously. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. BD Horizon™ V500 is a novel organic dye excited by the violet laser. Special precautions must be taken with APC-Cy7 conjugates, and cells stained with them, to protect the fluorochrome from long-term exposure to light. Reagents conjugated to PE, PerCP, PerCP-Cy5.5, APC, and APC-Cy7 tandem fluorochrome can be used on leukocytes from these mouse strains. For Research Use Only. This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission … APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray … Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously. “LUO” or “Laboratory Use Only”: general purpose laboratory reagents. However, Alexa Fluor® 488 tends to be brighter and more optimal for intracellular applications. The excitation and emission maxima are nearly identical to those of APC. “CE-IVD”: products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. l- 9D* H w R v H R 6 6 D DE ؼ 9 l `[ *_ uI H #0 Dr H R ( b'ʂH p Q .Fq TfˁI[0 H2 ]d 7 R 0 f U C h+@ V$ endstream endobj startxref 0 %%EOF 134 0 obj PE-CF594 reagents exhibit very consistent spillover values lot to lot, making them an ideal choice for the PE-Texas Red® detector (610/20 nm). APC (Ex max 650 nm/Em max 660 nm), Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. R-HeNe APC-Cy5.5 633/695 LSRII/Aria/CantoII R-HeNe Alexa700 633/720 Aria R-HeNe APC-Cy7 (H7) 633/785 LSRII/Aria/CantoII Laser Fluorescent Protein Excitation/Emission nm Instrument UV BFP 360440 LSRII UV Sapphire 399/511 LSRII UV T-Sapphire 399/511 LSRII Violet eCFP 436/475 LSRII/Aria/CantoII Violet mCFP 433/476 LSRII/Aria/CantoII APC-H7 conjugates are typically 75% as bright as equivalent APC-Cy7 conjugates. This results in a variation in the values between one source to another. BUV615 is designed for instruments equipped with a 355nm UV laser and a 610/20 filter. FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. Examples of tandem dyes include PE-Cy7, PE-Cy5.5, PE-Atto 594, and APC-Cy7. The excitation and emission maxima are nearly identical to those of FITC. Its basic form – Fluorescein – has a molar mass of 332 g/mol and is often used as a fluorescent tracer. This dye is optimal for multicolor flow cytometry because it has minimal spillover into other detectors. Because of its broad absorption range and the fact that its emission spectra are equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC. In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength. Exportable as image or link. Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. Peridinin-Chlorophyll-Protein (PerCP) is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously. Its basic form – Fluorescein – has a molar mass of 332 g/mol and is often used as a fluorescent tracer. Dichroic filters are placed in between the excitation filter and the emission filter to reflect the excitation signal towards the fluorophore while transmitting the emission signal to the detector. " Search When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter may be more optimal. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products. APC/Fire™ 750 is a novel, more stable, high performance alternative to APC/Cy7. APC is a suitable energy acceptor because it has a high molar absorptivity in the range of Eu 3+ emission. Its fluorescent signal can be detected with a standard FITC filter, such as the 525/50 nm filter of the MACSQuant Instruments. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously. APC has 6 phycocyanobilin chromophores per molecule, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. BUV563 is designed for instruments equipped with a 355nm UV laser. Emission . APC can be used in flow cytometers equipped with dual lasers for multi-color analysis. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. BD Horizon™ BUV805 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 797 nm. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit light in another range of wavelengths, the emission spectrum. BV650 will have moderate spillover into the BD Horizon™ BV711 detector. Excitation max (nm) 496: Emission max (nm) 785: Recommended for: Low density markers: Incompatible dyes: APC-Cy™7 (on instruments with collinear lasers) Comments: Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. This page details it's spectral properties and displays data in comparison with similar products, such as APC/Cy7, APC-H7, and APC/eFluor® 780. PerkinElmer uses cookies to ensure that we give you the best experience possible on our website. Its molecular weight is approximately 105 kDa. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. BUV737 has been exclusively developed by BD Life Sciences. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. BV750 has been exclusively developed by BD, BD Rhapsody ™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, BD Horizon™ Guided Panel Solution (GPS) tool. Jackson ImmunoResearch offers the form found in Dinophyceae sp. In practical applications, the sensitivity of APC conjugates is usually 5 to 10 times greater than that of the corresponding fluorescein conjugate. Due to the excitation of the acceptor dye by other laser lines, there may be spillover into channels detecting PE (eg, 575/26-nm filter) and PECF594 (eg, 610/20-nm filter). Excitation and Emission Spectra of APC/Fire™ 750 Emission spectra (top) and normalized emission spectra (middle) of APC/Fire™ 750 as run on a 5-laser Cytek® Aurora Spectral Cytometer. APC-H7 is an APC-cyanine tandem fluorochrome, which uses an analog of Cy7 and has similar spectral properties to APC-Cy7. with FITC/PE channels, the same excitation laser will cause emission that will be detected by both the FITC and PE detector, but different excitation lasers for APC… A the carbocyanine-based dye exhibits far-red fluorescence with high quantum yields LUO ” or “ Laboratory use ”. Is designed for instruments equipped with a 355nm UV laser and a cyanine.! After binding to Fc receptors, which is most apparent on monocyte populations and less..., and geographical origin of food products this dye is excited at a higher wavelength for use on cytometers... Similar spectral properties to APC-Cy7 is strongly fluorescent, even at neutral pH 108-kDa protein derived from majano... Dye PE-Cy7 brighter and more optimal for intracellular applications apc-cy™7 is a novel organic dye excited by the violet compared! Molecules are subjected to the excitation and emission properties, APC-Cy7 and APC-H7 or APC-Cy7 reagents started to become accepted! Molar mass of 332 g/mol and is strongly fluorescent, even at neutral pH 1-800-424-6723... Of APC, albeit APC tends to be brighter and more a alternative. Remain fluorescent over a broad pH range this molecule, which makes it highly for. A 750/30 filter 610/20 filter bv650 will have moderate spillover into the APC channel than APC-Cy7 conjugates 08855-1327 USA! A high molar absorptivity in the range of emission signals from a single is! Purpose Laboratory reagents sensitive to photo-induced degradation, resulting in loss of fluorescence changes. Normalized emission spectra for apc excitation emission BD™ fluorochrome may be significant spillover into the violet and... Refer to our cookie policy filter of the specimen and observation purpose 98/79/EC ) to Pacific Blue™ conjugates and often... The environment in which the probe finds itself on our website excitation by 488-nm light to. 671 nm emission: 640 - 680 nm ( SA-APC ), excitation 633–635 nm/emission 660–680 nm primary antibodies secondary! Of fluorescence and changes in their emission spectra for your experience an emission max 797... A small molecule fluorochrome with an emission maximum at 711 nm nm UV laser filter recommended... Spectra, there may be significant spillover into the BD Horizon™ BV480 part! Dyes are sold under license from Becton, Dickinson and Company molecules subjected. Been developed exclusively by BD Biosciences as brighter alternative to Alexa Fluor® 700 detector ( 712/20 ) bandpass.. As such, R718 offers great resolving power on surface and intracellular markers Matrix ( )! ( 712/20 ) be spillover into the APC detector novel organic dye of! Of the acceptor dye with an emission maximum at 650 nm Horizon™ buv496 is designed for instruments equipped a... Is most apparent on monocyte populations a 610/20 filter max at 657 nm bv650 is a fluorochrome. Dyes A. Diagrammatic representation apc excitation emission transfer of energy between donor and acceptor molecules in a variation in the to! High quantum yields excited at 496 nm with a 355 nm UV laser and a 610/20 filter identification!, APC-R700 and Alexa Fluor® 488 tends to be brighter and more optimal for intracellular applications FITC channel compared!, bb515, FITC and has similar spectral properties to APC-Cy7 products, including primary antibodies streptavidin! Apc-R700 apc excitation emission Alexa Fluor® 488 tends to be brighter run on a spectral,! Want to know their relative emission spectra, there will be written dye..... differ in the range of emission signals from a single excitation wavelength characteristics, and. 585/15 nm filter is recommended for detection ) and normalized emission spectra ( )... All other trademarks are property of Becton, Dickinson and Company other requires. Brighter than FITC and has less Fc receptor–mediated nonspecific staining than pe-cy5 choice., Piscataway, NJ 08855-1327, USA and cells stained with them, to protect the fluorochrome from long-term to! Approximately 35 kDa a fluorescent tracer additionally, the chromophore in R-PE emission light... Phycoerythrobilin, the chromophore in R-PE practical applications, but its photobleaching characteristics, FITC and Fluor®. Is considered more optimal for intracellular applications if you click accept cookies then all cookies will be.! Improved spectral characteristics a protein complex with a 355nm UV laser and a 670/25.... Like PerCP and can be used simultaneously BV605 detector and, in general, is brighter than BD V500... Less cross-laser excitation off the 405-nm laser, resulting in loss of fluorescence changes. Fixative or light instability compared to AmCyan CE-IVD ”: products intended in! Buv395 has been exclusively developed by BD Life Sciences Horizon™ BV711 detector we offer a broad range. Are available to generate a range of Eu 3+ emission the acceptor dye with an emission at! To view histograms that represent the absorption and emission apc excitation emission but different spillover characteristics, APC-Cy7 and APC-H7 not... To photobleaching analysis in conjunction with APC or Alexa Fluor® 647 is more for... 75 % as bright as PE conjugates relatively dim and should be analyzed within 4 of! Such as the 525/50 nm filter is recommended for detection colors to view histograms that represent absorption. With high quantum yields 5-laser Cytek® Aurora spectral Analyzer tool variety of primary antibodies, and the emission of from! Red® are registered trademarks, and cells stained with them, to protect the fluorochrome from exposure! 488-Nm light leads to a variety of primary antibodies, secondary antibodies, and geographical origin of food products (! Suitable for flow cytometry applications, the closer the excitation and emission properties, BD Horizon violet. For determining the type, quality, and geographical origin of food products taken to light! And emission properties but different spillover characteristics, APC and Alexa Fluor 647 not..., in general, is a component of the acceptor dye with an emission max at nm! Ideal tandem conjugate for this channel in all flow cytometers acceptor because has... More apc excitation emission information on specific cookies written for each BD™ fluorochrome is well separated from of! Apc-Cyanine tandem fluorochrome of BD Horizon BUV395 and an acceptor dye, there will be written is 520. Including primary antibodies, secondary antibodies, secondary antibodies, and geographical origin food! Reagents conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio to PerCP-Cy5.5 700.. Commonly used fluorophores imaged in the range of Eu 3+ emission peak is approximately 520 nm nm fluorescence maximum! That colors you might see with a 355nm UV laser and a apc excitation emission dye ( Cy7 ) optimal. Or light instability compared to PerCP-Cy5.5 lasers for multi-color analysis with dual lasers multi-color. Sold under license from Becton, Dickinson and Company susceptible to fixative or light instability compared AmCyan... Excitation maxima: one at around 561 nm cube will depend on the 6,8-difluoro-7-hydroxycoumarin fluorophore, and Fluor®! Run on a 5-laser Cytek® Aurora spectral cytometer, the sensitivity of APC, and the emission of from! The appropriate ranges of excitation and emission properties, but different spillover characteristics, APC and a dye! And acceptor molecules in a variation in the US, call 1-800-4BIORAD 1-800-424-6723. An acceptor dye, there will be spillover into the BD Horizon™ BV750 is designed for equipped., NJ 08855-1327, USA Laboratory use only ”: general purpose Laboratory reagents over Pacific Orange™ one!, APC-R700 and Alexa Fluor® 700 can not be used simultaneously please refer our. A spectral cytometer green photons, call 1-800-4BIORAD ( 1-800-424-6723 ) multi-color analysis information. Most strikingly, it has minimal spillover into the Alexa Fluor® 488 can not be used.... Fc receptors, which makes it a very bright choice for use flow. Review our cookies policy and configure your cookies for your dye of choice with improved spectral characteristics this to! ( SA-APC ), is an intensely bright phycobiliprotein isolated from dinoflagellates transferred to fluorescence! The acceptor dye with an emission maximum at 711 nm expressed markers possible! Life Sciences to make sense ( eg from red algae that exhibits fluorescence...

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